CUSTOM SERVICES
DNA methylation quantitation by Pyrosequencing
We offer DNA methylation quantitation by Pyrosequencing services, from assay design to data generation.
DNA methylation quantitation by Pyrosequencing is a method of determining DNA sequence based on sequencing by synthesis of bisulfite converted DNA. Unlike Sanger sequencing that relies on chain termination with dideoxynuclotides to identify nucleotide sequence. Pyrosequencing is designed to detect pyrophosphate release upon nucleotide incorporation. The DNA sequence is determined by light emitted upon incorporation of the next complementary nucleotide out of four possible nucleotides; dATP, dTTP, dCTP, dGTP. The intensity of the light emitted determines if more than one of these nucleotides exist in a row. The previous nucleotide is degraded before the next nucleotide letter is added for synthesis: allowing for the sequence of the DNA fragment to be revealed. Pyrosequencing is used in quantification of DNA methylation, multi-copy genes, ploidy levels, pooled DNA samples, heterozygosity, hematopoeitic chimerism, and mixed genotypes in heterogeneous samples (tumor and normal cells).
Order your pyrosequencing assay today
We offer DNA methylation quantitation by Pyrosequencing services, from assay design to data generation.
DNA methylation quantitation by Pyrosequencing is a method of determining DNA sequence based on sequencing by synthesis of bisulfite converted DNA. Unlike Sanger sequencing that relies on chain termination with dideoxynuclotides to identify nucleotide sequence. Pyrosequencing is designed to detect pyrophosphate release upon nucleotide incorporation. The DNA sequence is determined by light emitted upon incorporation of the next complementary nucleotide out of four possible nucleotides; dATP, dTTP, dCTP, dGTP. The intensity of the light emitted determines if more than one of these nucleotides exist in a row. The previous nucleotide is degraded before the next nucleotide letter is added for synthesis: allowing for the sequence of the DNA fragment to be revealed. Pyrosequencing is used in quantification of DNA methylation, multi-copy genes, ploidy levels, pooled DNA samples, heterozygosity, hematopoeitic chimerism, and mixed genotypes in heterogeneous samples (tumor and normal cells).
Order your pyrosequencing assay today
Ultrapure-Sterile Bulk Media and Reagent Manufacturing
Our team of highly trained scientists will manufacture any molecular biology reagent in bulk. Our capacity is in excess of 36,000 liters annually. We also manufacture microbiology media and plates.
Please call customer service 1-800-501-9558 or e-mail your order to orders@americanbioinnovations.com
Our team of highly trained scientists will manufacture any molecular biology reagent in bulk. Our capacity is in excess of 36,000 liters annually. We also manufacture microbiology media and plates.
Please call customer service 1-800-501-9558 or e-mail your order to orders@americanbioinnovations.com
Testing and Certifying Chemicals for Cell Culture Applications
American BioInnovations, validates cell culture ingredients for cell culture application. We provide certification and a rapid turn around time of 1 week upon receiving the chemicals for testing. Please contact as with your inquiry today, orders@americanbioinnovations.com
American BioInnovations, validates cell culture ingredients for cell culture application. We provide certification and a rapid turn around time of 1 week upon receiving the chemicals for testing. Please contact as with your inquiry today, orders@americanbioinnovations.com